Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

Blog Article

Summary: High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries.High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling.Here, we describe a protocol for epitope gtech brush bar and RNA-preserving dissociation of adult mouse brains and subsequent leukocyte purification and staining, which el reformador tequila anejo is adaptable to homeostatic and pathogenic brains.

Leukocyte preparation following this protocol permits exquisite single-cell surface protein and RNA profiling in applications including CyTOF and CITE-seq.For complete details on the use and execution of this protocol, please refer to Guldner et al.(2020) and Golomb et al.

(2020).